DMEM	500 ml
Sodium pyruvate	7.5 ml (100mM)
L-Glutamine	5 ml (200mM)
Pen/Strep	5 ml (10,000U/ml)
Non-essential amino acids	5 ml (MEM Non-Essential Amino Acids Solution (100×))
Fetal bovine serum	50 ml
Arginine Asparagine	5 ml (L-Arginine 1.16g, L-Asparagine 0.36g/ 100ml w/ddH2O)
Folic acid	5 ml (Folic acid 0.06g, 1N NaOH 0.5ml/ 100ml w/ddH2O)
Vitamins	5 ml (MEM vitamin mix solusion (100×)
2-mercaptoethanol	2 ml (of pre-diluted stock)
Gentamicin	1 ml (50mg/ml)