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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Biomaterials. 2018 Mar 29;169:61–71. doi: 10.1016/j.biomaterials.2018.03.049

Fig. 3. TSP-2 KO degradation products induce chemotactic effects.

Fig. 3

Degradation products were prepared by incubating decellularized skin in a pepsin solution. Degradation products were added to serum-free media at a concentration of 50 μg/mL, and chemotaxis toward them was tested in a Transwell. NIH/3T3 embryonic fibroblasts (A) and MC3T3-E1 preosteoblasts (B) migration significantly increased toward TSP-2 KO matrix than the pepsin control. WT display no greater chemotaxis than control. HUVECs (C) and RAW 264.7 macrophages (D) display no chemotactic activity toward either degradation product (n=3).