Intact slabs of constructs were implanted subcutaneously for 14 days, scale bar = 100 μm (A), and TSP-2 KO constructs display increased cell penetration into the matrix. Implanted silicone trays are out of frame but reside below and to the sides of the implanted ECM; ECM is just below the dermis (n=4) (B). CDM was analyzed as an in vitro system to probe cell migration through WT and TSP-2 KO matrix (C), and it was found that TSP-2 null ECM is more permissive to both WT and db/db fibroblast migration, n=5 (D–E). Scale bars = 100 μm. Results are given as mean + SEM, *p<0.05, **p<0.01.