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Optimization of the plasma dilution ratio for measuring acetylcholinesterase (AChE) activity. AChE activity was measured as a plasma dilution ratio to find the optimal plasma dilution conditions. When plasma was diluted 1:50, AChE activity was measured as 17.0 ± 0.1 mU/ml (A). Inhibition of AChE activity was also observed in a donepezil concentration-dependent manner at a dilution ratio of 1:50 (B). Data represent the means ± SEM.
