Fig. 4.

Gomisin G induced cyclin D1 degradation in MDA-MB-231 cells. (A) MDA-MB-231 and (B) T47D cells were treated with 10 µM of Gomisin G for 72 h. The expression of cyclin D1 was determined with immunoblotting. β-actin was used as a loading control. (C) MDA-MB-231 cells were treated with 10 µM of Gomisin G for 42 h followed by 10 µM of MG132 for 6 h. The expression of cyclin D1 and ubiquitin was determined with immunoblotting. β-actin was used as a loading control.