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(A) Five-fold serially diluted cultures of the indicated strains were plated on rich media (YE5S) at 32°C. (B) Growth of cells harboring a temperature sensitive allele of cnp1+ (cnp1-1) with and without overexpressed (nmt41-controlled) Ams2 was assessed with five-fold serially diluted cultures incubated at 25°C and 32°C. (C) Viability assay was performed as described in (A).
