Key resources table.
| Reagent type (species) or resource | Designation | Source | Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound | Thiabenzadole | Sigma-Aldrich | Cat#T8904 | |
| Chemical compound | Methyl methanesulfonate | Sigma-Aldrich | Cat#66-27-3 | |
| Chemical compound | 5-Fluoroorotic Acid | US Biological | Cat#207291-8-4 | |
| Chemical compound | nourseothricin dihydrogen sulfate | US Biological | Cat#N5374-74 | |
| Chemical compound | G418 sulfate (Geneticin) | Invitrogen | Cat#11811031 | |
| Chemical compound | Hygromycin B | Invitrogen | Cat#10687010 | |
| Chemical compound | Hydroxyurea | Sigma-Aldrich | Cat#H8627 | |
| Reagent for microscopy | glass-bottom dish | MatTek | Cat#P35G-1.5–14 C | |
| Reagent for microscopy | GFP booster_ATTO488 | Chromotek | Cat#gba488-100 | |
| Reagent for microscopy | RFP booster_ATTO594 | Chromotek | Cat#rba594-100 | |
| Reagent for microscopy | Vectashield | Vector Labs | Cat#H-1000 | |
| Reagent for microscopy | 16% Paraformaldehyde aqueous | Electron Microscopy Sciences | Cat#15710 | |
| Reagent for microscopy | 8% glutaraldehyde | Electron Microscopy Sciences | Cat#16019 | |
| Reagent for microscopy | Zymolyase-100T | MP Biomedicals | Cat#320932 | |
| Reagent for ChIP | anti-H3K9Me2 ChIP Grade | Abcam | Cat#mAbcam1220 | |
| Reagent for ChIP | Complete, EDTA-free Protease inhibitor cocktail (Roche) | Sigma-Aldrich | Cat#11873580001 | |
| Reagent for ChIP | Phenylmethylsulfonyl fluoride | Sigma-Aldrich | Cat#P7626 | |
| Reagent for ChIP | Zirconium beads, 0.7 mm diameter | BioSpec Products | Cat#11079107zx | |
| Reagent for ChIP | Dynabeads Protein G | Invitrogen | Cat#10003D | |
| Reagent for ChIP | ChIP Elute Kit | Clontech | Cat#634887 | |
| Reagent for ChIP | milliTUBE 1 ml AFA Fiber | Covaris | Cat#520130 | |
| Reagent for ChIP | DNA SMART ChIP-Seq Kit | Clontech | Cat#634865 | |
| Reagent for ChIP | Agencourt AMPure XP | Beckman Coulter | Cat#A63880 | |
| Reagent for ChIP | NEBNext Library Quant Kit | New England BioLabs | Cat#E7630S | |
| Strain (Schizosaccharomyces pombe) |
h+ ade6 M216 his3-D1 leu1-32 ura4-D18 | Lab stock | JCF109 | |
| Strain (Schizosaccharomyces pombe) | h- ade6-M210 leu1-32 ura4-D18 lys1+::GFP-bqt4 | This study | JCF11923 | Plasmid pCSS18 (Chikashige et al., 2009) was integrated at lys1 locus. |
| Strain (Schizosaccharomyces pombe) | h90 man1-GFP-kanMX6 aur1R-mCherry-bqt4 bqt4Δ: :natMX6 leu1-32 ura4-D18 | This study | JCF1266 | |
| Strain (Schizosaccharomyces pombe) | h90 lem2-GFP-kanMX6 aur1R-mCherry-bqt4 bqt4Δ: :natMX6 ura4-D18 | This study | JCF1268 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-kanMX6 man1-tdTomato-hygMX6 leu1-32 ura4-D18 | This study | JCF18885 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-kanMX6 hht1-mRFP-hygMX6 | This study | JCF12191 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-kanMX6 hht1-mRFP-hygMX6 bqt4::natMX6 | This study | JCF12201 | |
| Strain (Schizosaccharomyces pombe) | ura4-D18 lem2-GFP-KanMX6 | This study | JCF12148 | |
| Strain (Schizosaccharomyces pombe) | ura4-D18 lem2-GFP-KanMX6 bqt4::nat | This study | JCF12157 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-kanMX6 sid4-mCherry-natMX6 aur1R-Pnda3-mCherry-atb2 | This study | JCF14537 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-kanMX6 sid4-mCherry-natMX6 aur1R-Pnda3-mCherry-atb2 csi1::his3+ | This study | JCF14540 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-kanMX6 bqt4::natMX6 csi1::ura4+ | This study | JCF14573 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-kanMX6 taz1-mCherry-natMX6 csi1::his3+ | This study | JCF14535 | |
| Strain (Schizosaccharomyces pombe) | h- taz1-mCherry-natMX6 lys1+::GFP-bqt4 | This study | JCF14578 | |
| Strain (Schizosaccharomyces pombe) | h- taz1-mCherry-natMX6 man1-GFP-KanMX6 | This study | JCF14565 | |
| Strain (Schizosaccharomyces pombe) | h+ taz1-mCherry-natMX6 lem2-GFP-KanMX6 | This study | JCF14532 | |
| Strain (Schizosaccharomyces pombe) | h+ mis6-mCherry-hygMX6 lem2-GFP-KanMX6 | This study | JCF14543 | |
| Strain (Schizosaccharomyces pombe) | h+ mis6-mCherry-hygMX6 man1-GFP-KanMX6 | This study | JCF14533 | |
| Strain (Schizosaccharomyces pombe) | ade6-210 leu1-32 lys1-131 ura4-D18 sod2::kanr-ura4+-lacOp his7+::lacI-GFP cut11-RFP-hygMX6 | This study | JCF10756 | |
| Strain (Schizosaccharomyces pombe) | ade6-210 leu1-32 lys1 + ura4- D18 sod2::kanr-ura4+-lacOp his7+::lacI-GFP cut11-RFP-hyg bqt4::nat | This study | JCF10771 | |
| Strain (Schizosaccharomyces pombe) | ade6-210 leu1-32 lys1-131 ura4-D18 sod2::kanr-ura4+-lacOp his7+::lacI-GFP cut11-RFP-hyg lem2::nat | This study | JCF12202 | |
| Strain (Schizosaccharomyces pombe) | his2::kanMX6-ura4+-lacOp his7+::lacI-GFP cut11-RFP-hygMX6 | This study | JCF10732 | |
| Strain (Schizosaccharomyces pombe) | his2::kanMX6-ura4+-lacOp his7+::lacI-GFP cut11-RFP-hygMX6 bqt4::natMX6 | This study | JCF10740 | |
| Strain (Schizosaccharomyces pombe) | cut3+::lacOp his7+::lacI-GFP cut11-RFP-hygMX6 | This study | JCF10755 | |
| Strain (Schizosaccharomyces pombe) | cut3+::lacOp his7+::lacI-GFP cut11-RFP-hygMX6 bqt4::nat | This study | JCF11930 | |
| Strain (Schizosaccharomyces pombe) | taz1-mCherry-nat pLT1-2 (nmt1:Ish1-GFP-LEU2) | This study | JCF10701 | |
| Strain (Schizosaccharomyces pombe) | taz1-mCherry-nat bqt4::hygMX6 pLT1-2 (nmt1:Ish1-GFP-LEU2) | This study | JCF10723 | |
| Strain (Schizosaccharomyces pombe) | pot1-mRFP:kanMX6 cut11-3xPK-GFP:ura4 + leu1-32 ura4-D18 (WT) | Lab stock | JCF3649 | |
| Strain (Schizosaccharomyces pombe) | cut11-RFP-hyg tpz1-GFP-Kan taz1::ura4+ | This study | JCF12293 | |
| Strain (Schizosaccharomyces pombe) | cut11-RFP-hyg tpz1-GFP-Kan bqt4::nat | This study | JCF12291 | |
| Strain (Schizosaccharomyces pombe) | cut11-RFP-hygMX6 tpz1-GFP:kanMX6 bqt4::nat taz1::ura4+ | This study | JCF12290 | |
| Strain (Schizosaccharomyces pombe) | his2::kanMX6-ura4+-lacOp his7+::lacI-GFP cut11-RFP-hyg bqt4::nat swi6::leu2+ | This study | JCF10807 | |
| Strain (Schizosaccharomyces pombe) | his2::kanMX6-ura4+-lacOp his7+::lacI-GFP cut11-RFP-hyg swi6::leu2+ | This study | JCF 12279 | |
| Strain (Schizosaccharomyces pombe) | h- swi7-GFP::kanMX6 taz1-mCherry-natMX6 | This study | JCF11905 | |
| Strain (Schizosaccharomyces pombe) | swi7-GFP::kanMX6 taz1-mCherry-nat bqt4::hygMX6 | This study | JCF14504 | |
| Strain (Schizosaccharomyces pombe) | h- bqt4::nat ade6-M210 his3-D1 leu1-32 ura4-D18 | This study | JCF11933 | |
| Strain (Schizosaccharomyces pombe) | h+ ade6 M216 trt1::hygMX6 | Jain et al. (2010) | JCF6858 | Circular survivor |
| Strain (Schizosaccharomyces pombe) | h+ ade6 M210 clr4::kanMX6 | Lab stock | JCF3113 | |
| Strain (Schizosaccharomyces pombe) | h- clr4::kanMX6 bqt4::hygMX6 | This study | JCF10795 | |
| Strain (Schizosaccharomyces pombe) | h+ swi7-GFP-kanMX6 man1-GBP-mCherry-hph | This study | JCF11950 | GBP plasmids were gifts from Masamitsu Sato, University of Tokyo |
| Strain (Schizosaccharomyces pombe) | h- swi7-GFP-kanMX6 | Natsume et al. (2008) | JCF10835 | |
| Strain (Schizosaccharomyces pombe) | h+ swi7-GFP-kanMX6 man1-GBP-mCherry-hph bqt4::naMX6 | This study | JCF14517 | |
| Strain (Schizosaccharomyces pombe) | h90 otr1R::ade6 tel1L::his3 tel2L::ura4 ade6-210 his3-D1 leu1-32 ura4-D18 | Nimmo et al., 1998 | JCF6712 | Spike strain in ChIP-seq experiments. Used for telomere silencing assay |
| Strain (Schizosaccharomyces pombe) | h- dcr1::ura4+ | This study | JCF12179 | |
| Strain (Schizosaccharomyces pombe) | h+ ade6 M216 his3-D1 leu1-32 ura4-D18 bqt4::natMX6 | This study | JCF14512 | |
| Strain (Schizosaccharomyces pombe) | h- leu1-32 ura4-D18 T2R1-4137::ura4+ | Kanoh et al. (2005) | JCF10833 | |
| Strain (Schizosaccharomyces pombe) | h- swi6::LEU2 leu1-32 ura4-D18 T2R1-4137::ura4+ | Kanoh et al. (2005) | JCF10830 | |
| Strain (Schizosaccharomyces pombe) | h- leu1-32 ura4-D18 T2R1-4137::ura4 + bqt4::natMX6 | This study | JCF10833, 10834 | |
| Strain (Schizosaccharomyces pombe) | h- swi6::LEU2 leu1-32 ura4-D18 T2R1-7921::ura4+ | Kanoh et al. (2005) | JCF10829 | |
| Strain (Schizosaccharomyces pombe) | h- leu1-32 ura4-D18 T2R1-7921::ura4 + bqt4::natMX6 | This study | JCF11998-12002 | |
| Strain (Schizosaccharomyces pombe) | h- leu1-32 ura4-D18 T2R1-7921::ura4+ | Kanoh et al. (2005) | JCF10832 | |
| Strain (Schizosaccharomyces pombe) | h+ lem2::kanMX6 | This study | JCF12178 | |
| Strain (Schizosaccharomyces pombe) | h90 epe1::natMX6 | This study | JCF10787 | |
| Strain (Schizosaccharomyces pombe) | epe1::natMX6 lem2::kanMX6 | This study | JCF12204 | |
| Strain (Schizosaccharomyces pombe) | h+ lem2::kanMX6 dcr1::ura4 | This study | JCF12182 | |
| Strain (Schizosaccharomyces pombe) | h- epe1::naMX6t dcr1::ura4 | This study | JCF12209 | |
| Strain (Schizosaccharomyces pombe) | epe1::natMX6 dcr1::ura4 lem2::kanMX6 | This study | JCF12211 | |
| Strain (Schizosaccharomyces pombe) | h+ cnp1-1 leu1 Rep41-ams2+[leu1+] | NBPR | JCF14580 | Yeast Genetic Resource Center (YGRC), Graduate School of Science, Osaka City University |
| Strain (Schizosaccharomyces pombe) | lem2::natMX6 cnp1-1 leu1 Rep41-ams2+[leu1+] | This study | JCF14588 | |
| Strain (Schizosaccharomyces pombe) | h+ cnp1-1::ura4 + ura4-D18 | NBPR | JCF10266 | Yeast Genetic Resource Center (YGRC), Graduate School of Science, Osaka City University |
| Strain (Schizosaccharomyces pombe) | h- bqt4::nat leu1-32 ura4-D18 his3-D1 ade6-M210 | This study | JCF11933, 11934 | |
| Strain (Schizosaccharomyces pombe) |
epe1::natMX6 dcr1::ura4 +
bqt4::hygMX6 |
This study | JCF12240 | |
| Strain (Schizosaccharomyces pombe) | h- bqt4::hygMX6 epe1::natMX6 | This study | JCF10811 | |
| Strain (Schizosaccharomyces pombe) | h+ bqt4::hygMX6 dcr1::ura4+ | This study | JCF12180 | |
| Strain (Schizosaccharomyces pombe) | h- ade6-M210 leu1-32 ura4-D18 his3-D1 sid4-GFP-kanMX6 dh1L-tetO-ura+tetR-mCherry-nat | This study | JCF14534 | |
| Strain (Schizosaccharomyces pombe) | h- his7+::adh13pr-lacI-CFP C12::kanR-ura4+-lacOp sid4-mRFP-natR ade6-210 leu1-32 ura4-D18 lys1-131 | This study | JCF18924 | |
| Strain (Schizosaccharomyces pombe) | ade6-210 leu1-32 lys1-131 ura4-D18 sod2::kanr-ura4+-lacOp his7+::lacI-GFP cut11-RFP-hyg clr4::nat | This study | JCF11961 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-Kan bqt4::nat pcp1-RFP-hph | This study | JCF12189 | |
| Strain (Schizosaccharomyces pombe) | lem2-GFP-Kan pcp1-RFP-hph | This study | JCF12190 | |
| Strain (Schizosaccharomyces pombe) | man1-GFP-kan ura4-D18 | This study | JCF12153 | |
| Strain (Schizosaccharomyces pombe) |
h+ man1 GFP-kan bqt4::nat
ura4-D18 |
This study | JCF12163 | |
| Strain (Schizosaccharomyces pombe) | h- ade6-M210 his3-D1 leu1-32 ura4-D18 nup107-GFP:ura4+ | This study | JCF11770 | |
| Strain (Schizosaccharomyces pombe) | nup107-GFP:ura4 + bqt4::nat | This study | JCF12177 | |
| Strain (Schizosaccharomyces pombe) | cut11-3xPK-GFP:ura4 + leu1-32 ura4-D18 | Lab stock (from Nurse lab) |
JCF2924 | |
| Strain (Schizosaccharomyces pombe) | h+ cut11 GFP-kan bqt4::nat ura4-D18 | This study | JCF12176 | |
| Strain (Schizosaccharomyces pombe) | h- bqt4::nat leu1-32 ura4-D18 his3-D1 ade6-M210 aur1R-GFP-bqt4ΔTM | This study | JCF12048, 12049 | |
| Strain (Schizosaccharomyces pombe) | lys1+-GFP-bqt4 bqt4::nat ura4-D18 ade6-M210 | This study | JCF11958 | |
| Strain (Schizosaccharomyces pombe) | imr1R(Nco1)::ura4 + swi7 GFP-kanR ura4-D18 leu1-32 ade6-M216 | This study | JCF18888 | |
| Strain (Schizosaccharomyces pombe) | imr1R(Nco1)::ura4 + swi7 GFP-kanR ura4-D18 leu1-32 | This study | JCF18890 | |
| Strain (Schizosaccharomyces pombe) | imr1R(Nco1)::ura4 + ura4-D18 leu1-32 ade6-M216 | This study | JCF18892 | |
| Strain (Schizosaccharomyces pombe) | imr1R(Nco1)::ura4 + ura4-D18 leu1-32 | This study | JCF18893 | |
| Strain (Schizosaccharomyces pombe) | h+ his3D1leu1-32 ade6 | Lab stock | JCF901 | |
| Strain (Schizosaccharomyces pombe) | h- rad11-mCherry-kanR leu1-32 ura4-D18 ade6 | Lab stock | JCF1234 | |
| Strain (Schizosaccharomyces pombe) | swi7-GFP::kanMX6 man1-GBP-mCherry-hph rad11-mCherry-Kan | This study | JCF14598 | |
| Strain (Schizosaccharomyces pombe) | taz1-mTurquoise2-natR man1-GBP-mCh-hphR ura4-D18 his3-D1 ade6 | This study | JCF18936 | |
| Strain (Schizosaccharomyces pombe) | taz1-mTurquoise2-natR swi7-GFP-kanR man1-GBP-mCh-hphR ura4-D18 his3-D1 ade6 | This study | JCF18939 | |
| Strain (Schizosaccharomyces pombe) | h90 otr1R::ade6 + tel1L::his3 + tel2L::ura4 + ade6-210 his3-D1 leu1-32 ura4-D18 | Lab stock | JCF6712 | |
| Strain (Schizosaccharomyces pombe) |
h90 otr1R::ade6 + tel1L::his3 + tel2L::ura4 + clr4::kanMX ade6-210 his3-D1 leu1-32
ura4-D18 |
Lab stock | JCF6715 | |
| Strain (Schizosaccharomyces pombe) | h90 otr1R::ade6 + tel1L::his + 3 tel2L::ura + 4 bqt4::nat ade6-210 his3-D1 leu1-32 ura4-D18 | This study | JCF10763 | |
| Other | YES225 | Sunrise Science Products |
Cat#2011–500 | |
| Other | PMG | Sunrise Science Products |
Cat#2060–500 | |
| Other | EMM | Sunrise Science Products |
Cat#2005–500 |