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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Comp Neurol. 2017 Apr 21;525(10):2358–2375. doi: 10.1002/cne.24209

Fig. 6. 5-HT3A-positive fibers are found in some brainstem regions where CGRP immunoreactivity is also found within the nTS and adjacent DMSp5.

Fig. 6

For each image-drawing pair: Left: photomicrographs of 5-HT3A GFP (green) and CGRP (magenta) immunoreactivity. The white dotted line demarcates the boundary of the nTS. DMSP5: dorsomedial nucleus of the descending trigeminal complex. Right: Spatial distribution of CGRP and 5-HT3A labeled pixels that are 2X standard deviation of background (identified using a customized MATLAB program). Labeled pixels that overlap are shown as black. The solid black outline indicates the boundaries of the tissue; whereas the black dotted line demarcates the boundary of the nTS.