Figure 5.

Comparison of the accumulation levels of normal and Met-modified glycinins in individual rice seeds by dot immunoblotting. Extracts (10 μg of proteins) of matured seeds from transgenic rice plants 11[pGluB1Gly] (A) and 417[pGluB1GlyIV] (C) were analyzed by immunoblotting. Between 0.001 and 1 μg of purified glycinin mixed with 10 μg of proteins of seed extract from nontransgenic rice plant was used as standard. The number below each dot indicates an individual seed. Frequencies of the accumulation levels of the expressed proteins in the individual seeds of 11[pGluB1Gly] (B) and 417[pGluB1GlyIV] (D) were calculated from dot blots.