Figure 6.

Analysis of the assembly of the normal glycinins accumulated in the seeds of the transgenic rice plant. Seed extracts were subjected to centrifugation on a 12-mL, 10% to 30% (w/v) linear Suc density gradient and analyzed by SDS-PAGE in the absence (A and C) or presence (B and D) of ME followed by immunoblotting. Sedimentation is from left to right. Sedimentation standards in Svedberg units are given. Arrowheads indicate the positions of recombinant proglycinin (Pro) synthesized in E. coli (lane P) and native mature glycinin (A-B; lane M). A and B, Nontransgenic rice; C and D, 11[pGluB1Gly].