Figure 7.

Analysis of the formation of hybrid-11S storage protein complexes between glycinin and glutelin in seeds of transgenic rice plants. The same Suc density gradient fractions depicted in Figure 6 were analyzed by SDS-PAGE in the absence (A and C) and presence (B and D) of ME followed by immunoblotting using anti-proglutelin GluA-1 serum. Sedimentation is from left to right. Sedimentation standards are given in Svedberg units. Arrowheads indicate the positions of recombinant proglutelin (Pro) (lane B) and the native acidic polypeptide (A) of glutelin. One microgram each of recombinant proglycinin (lane P) and native mature glycinin (lane M) was electrophoresed as negative controls. A and B, Nontransgenic rice; C and D, 11[pGluB1Gly].