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Solubility analysis of glycinins accumulated in the seeds of transgenic rice plants. Seed proteins were extracted six successive times with saline solution followed by three successive times with lactic acid solution. Each saline extract (lanes 1–6) and a mixture of the lactic acid solution extract (lane 7) were electrophoresed in the absence of ME and then analyzed by immunoblotting using anti-glycinin serum. A-B denotes the position of the native mature glycinin (lane M).
