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Binding of C. roseus PLC to Suc-loaded vesicles. Ten micrograms of PLC was incubated with a range of concentrations of PIP2-containing vesicles (PIP2:PC:PS, 3:70:27 mol/mol), followed by ultracentrifugation as described in Methods. PLC activity remaining in the supernatant was assayed against PIP2 and compared with vesicle-free controls in which the activity was 10 pmol/min.
