Skip to main content
. Author manuscript; available in PMC: 2019 May 2.
Published in final edited form as: Neuron. 2018 Apr 19;98(3):547–561.e10. doi: 10.1016/j.neuron.2018.03.043

Figure 7. Calhm3 is Selectively Expressed in Type II TBCs.

Figure 7

(A-C) Double-label in situ hybridization of Calhm3 in gustatory tissues. Calhm3 mRNA is expressed in a subset of TBC of circumvallate (CvP) and fungiform (FuP) papillae and palate but is absent in taste buds of Pou2f3–/– mice lacking type II cells. (A) Fluorescence and immunohistochemical double-label in situ hybridization directly illustrates cellular co-expression of Calhm3 and Trpm5 in CvP taste buds. Calhm3 expression is absent from Trpm5-negative cells. Tas1r3 is expressed in a subset of Calhm3-expressing CvP TBCs. Calhm3 is expressed in the same cells that express Calhm1. (B) Calhm3 mRNA expressed in a subset of cells in taste buds of circumvallate (CvP) and fungiform (FuP) papillae and palate of WT mice is absent in taste buds of Pou2f3–/– mice lacking type II cells. (C) In CvP taste bud cells from Calhm3–/– mice, Calhm3 expression is absent, whereas Calhm1 expression is normal. Scale bar, 50 μm.

See also Figure S8.