Plasmid maps of pATAG3 and pATAG4. These binary
plasmids contain between the T-DNA borders the nptII
gene under control of the nopaline synthase (pnos)
promoter and the octopine synthase 3′ termination and polyadenylation
signals (3′ocs) (pATAG3 and pATAG4) and
the Escherichia coli gus gene (Jefferson, 1987) with the
potato st-l1 intron (gusint; Vancanneyt
et al., 1990) under the control of the CaMV 35S promoter
(p35S) and the nopaline synthase 3′ processing and
polyadenylation signals (3′nos) (pATAG3
only). pBR, Origin of replication; pVS1, stability and replication
functions of the Pseudomonas aeruginosa pVS1 plasmid
(Deblaere et al., 1987); Sm/Sp, spectinomycin and streptomycin
resistance locus; RB and LB, right and left border repeat of the T-DNA.
Single-cutting restriction enzymes are shown outside of the plasmids.