Schematic representation of the
arc5-I genomic clone and the T-DNA of the binary vectors
pATARC4-E, pATARC4-BM, and pATARC3-B1b. Coding regions are indicated by
arrows and 5′ and 3′ flanking regulatory sequences as blocks. Numbers
correspond with positions in the arc5-I gene relative to
the translation start site. Regions upstream of position −1,835 and
downstream of position +2,310 were not sequenced. E,
EcoRI site; B, BamHI site; M,
MunI-site; RB and LB, right and left border repeat of
the T-DNA; NPTII,
pnos-nptII-3′ocs chimeric
gene; GUS, the
p35S-gusint-3′nos chimeric
gene. pATARC3-B1b contains the BamHI fragment of the
arc5-I genomic clone, whereas pATARC4-E and pATARC4-BM
contain the complete genomic fragment and the
BamHI/MunI fragment, respectively.