Figure 2.
Schematic representation of the arc5-I genomic clone and the T-DNA of the binary vectors pATARC4-E, pATARC4-BM, and pATARC3-B1b. Coding regions are indicated by arrows and 5′ and 3′ flanking regulatory sequences as blocks. Numbers correspond with positions in the arc5-I gene relative to the translation start site. Regions upstream of position −1,835 and downstream of position +2,310 were not sequenced. E, EcoRI site; B, BamHI site; M, MunI-site; RB and LB, right and left border repeat of the T-DNA; NPTII, pnos-nptII-3′ocs chimeric gene; GUS, the p35S-gusint-3′nos chimeric gene. pATARC3-B1b contains the BamHI fragment of the arc5-I genomic clone, whereas pATARC4-E and pATARC4-BM contain the complete genomic fragment and the BamHI/MunI fragment, respectively.