Skip to main content
. 1999 Aug;120(4):1095–1104. doi: 10.1104/pp.120.4.1095

Figure 3.

Figure 3

SDS-PAGE on crude protein extracts of transgenic P. acutifolius and Arabidopsis seeds. Proteins were visualized with Coomassie Blue staining. A, P. acutifolius seed proteins extracted from nontransgenic (wild-type) NI576 (lane WT) plants, transgenic plants containing arc5-I (lanes A1 and A2), and transgenic plants containing arc5-II (lanes B1 and B2). Lane ARC5 contains arcelin-5 proteins purified from the P. vulgaris genotype G02771 (from top to bottom, Arc5a, Arc5b, and Arc5c, respectively), and lane M contains the marker proteins. The molecular masses are indicated on the left in kD. B, Crude protein extracts of Arabidopsis seeds of the nontransformed Columbia-0 genotype (lane 1) and of transgenic lines BM-410 (lane 2) or E-103 (lane 3). Lane 4 contains arcelin-5 proteins purified from the P. vulgaris genotype G02771, and lane M contains the marker proteins. The molecular masses are indicated on the left in kD.