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Quantification of Arc5a by western blot analysis in transgenic Arabidopsis seeds. Lanes contain 1 μg of protein of crude extracts of seeds of transgenic lines harboring the T-DNA of pATARC4-E (E; lanes 1–3) or the T-DNA of pATARC4-BM (BM; lanes 4–8). Lanes 9, 10, 11, 12, and 13 contain a dilution series of purified arcelin-5 proteins: 150, 100, 75, 50, and 25 ng were loaded, respectively.
