Table II.
Line | Copies | Arc5 Protein
Level
|
mRNA Ratio arc5-I/at2S | |
---|---|---|---|---|
Hemizygous | Homozygous | |||
n | ||||
P. acutifolius | ||||
B1b-27a | 2 (IRR) | 5.8 ± 1.5 | 16.2 ± 0.6 | |
B1b-8a | 2 (IRR) | 5.6 ± 0.6 | 23.8 ± 2.6 | |
B52b-44.4 | 1 | 6.3 ± 0.6 | 15.7 ± 1.4 | |
B52b-44.X | 1 | 6.7b | 25.4 ± 7.0 | |
Arabidopsis | ||||
BM-406 | 1 | ND | 5.3 ± 1.0 | 2.3 |
BM-301 | 1 | 6.8 ± 0.8 | 5.4 ± 1.6 | ND |
BM-409 | 1 | 5.2 ± 0.2 | 6.2 ± 1.0 | ND |
BM-410 | 1 | 6.5 ± 1.2 | 7.7 ± 1.0 | 10.5 |
BM-306 | 1 | 6.0 ± 0.1 | 8.1 ± 2.2 | ND |
BM-304 | 2 (IRR) | 1.3 ± 0.3 | 1.0 ± 0.1 | ND |
BM-414 | 2 (IRR) | 2.2 ± 0.1 | 1.2 ± 0.6 | 0.02 |
BM-402 | 2 (IRR) | 1.2 ± 0.1 | 0.8 ± 0.6 | ND |
BM-416 | ≥3 | 3.5 ± 0.7 | 1.3 ± 0.5 | ND |
BM-401 | ≥3 | 2.8 ± 0.0 | 2.5 ± 2.1 | ND |
BM-303 | ≥3 | 8.8 ± 1.5 | 5.0 ± 0.5 | ND |
BM-412 | ≥3 | 11.7 ± 0.0 | 14.3 ± 0.9 | 6.6 |
E-307 | 1 | 7.9 ± 0.8 | 8.6 ± 2.0 | ND |
E-402 | 1 | ND | 9.7 ± 0.2 | ND |
E-313 | 1 | 7.3 ± 0.6 | 9.8 ± 2.3 | 2.7 |
E-105 | 1 | 9.5 ± 0.9 | 11.9 ± 0.1 | ND |
E-103 | 1 | 8.1 ± 0.3 | 14.7 ± 0.1 | 2.0 |
E-305 | 2 (IRR) | 9.6 ± 0.4 | 14.1 ± 2.7 | 2.3 |
E-411 | ≥3 | 1.6 ± 1.0 | 3.2b | ND |
E-102 | ≥3 | 4.9 ± 0.3 | 10.8 ± 4.2 | 2.0 |
E-306 | ≥3 | 7.3 ± 2.4 | 13.0 ± 1.2 | ND |
Transgenic lines marked with B1b, B52b, BM, and E contain T-DNA inserts from pATARC3-B1b, pATARC3-B52b, pATARC4-BM, and pATARC4-E, respectively. In case of multiple copies, the organization (when known) is indicated between brackets. IRR, Inverted repeat over the right T-DNA border. The Arc5 protein level is indicated as percentage of total extractable protein of seeds of hemizygous or homozygous transgenic plants. Values are followed by the sd. ND, Not determined due to limited amounts of seeds available. In case of P. acutifolius, measurements were performed on aliquots of one hemizygous seed and thus really reflect the Arc5 level in a hemizygous seed. In contrast, in Arabidopsis, measurements were performed on an aliquot of approximately 500 seeds harvested from a hemizygous plant, thus representing the level in a mixture of homozygous transformed, homozygous nontransformed and hemizygous seeds.
These lines showed small deletions at the left and right border of the T-DNA inserts.
Due to the limited number of seeds available quantification was only performed once and thus no sd could be calculated.