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. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: Nat Genet. 2018 Apr 23;50(5):708–717. doi: 10.1038/s41588-018-0105-0

Figure 5. Copy number variant driver genes located on the potential double minute (DM) regions.

Figure 5

A. 66 tumors (33 P, 33 R) from 38 patients were predicted to contain at least one ecDNA that was detected with either copy number based or Amplicon Architect methods. Amongst these, 44 driver gene harboring ecDNAs were predicted in 25 primary tumors, of which 32 were also detected in the matching recurrent tumors. B. Left panel: DNA copy number and genomic rearrangements at predicted ecDNA loci that were predicted with the copy number based approach. Right panel: Representative FISH images in FFPE tissue sections showing amplification of EGFR, MET and MYC in (red) and control chromosomal probes (green). Fifty nuclei were examined per sample. Scale bars, 5 μM. C. DNA copy number based predictions of extrachromosomal DNA segments validated using whole genome or RNA sequencing.