Fig. 2.

Macrophage-specific expression of WASP is critical for the regulation of T-cell-transfer-induced colitis. Naive CD4⁺CD25⁻CD45RB^hi T cells (3–5 × 10⁵) from wild-type (WT) mice were transferred i.p. into Rag1^−/−, Was^mDelRag1^−/− and Was^−/−Rag1^−/− mice. a Mean ± SEM of percent initial body weight after transfer (% initial body weight). Data are cumulative of four independent experiments (Rag^−/− n = 13, includes Exp 1, 2, 3, 4; Was^mDelRag1^−/− n = 17, includes Exp 1, 2, 3, 4, Was^−/−Rag1^−/− n = 7, includes Exp 3, 4). Difference was not significant between Was^mDelRag1^−/− versus Was^−/−Rag1^−/−cohorts. **p < 0.01, ****p < 0.0001 (two-way ANOVA). b Representative photomicrographs of H&E-stained colonic section and histological score after naive T-cell transfer. Scale bars: 200 μm. c Colon length at 6 weeks post transfer (Rag^−/− n = 8; Was^mDelRag1^−/− n = 9). d Cytokines expression in colonic homogenates at 6 weeks post transfer. Data are cumulative of two independent experiments (Rag^−/− n = 6; Was^mDelRag1^−/− n = 6). e Absolute number and frequency of TNF⁺, IFN-γ⁺, IL-17A⁺ and IFN-γ⁺IL-17A⁺ helper T cells in the LP was determined by flow cytometry (Rag^−/− n = 9; Was^mDelRag1^−/− n = 9). Data are cumulative of three independent experiments. f Percentage of Treg cells (CD45⁺TCRβ⁺CD4⁺CD25⁺FoxP3⁺) in the LP was determined by flow cytometry (Rag^−/− n = 5; Was^mDelRag1^−/− n = 5). Data are cumulative of two independent experiments. Data shown in b–f are mean ± SEM and P-value was obtained by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001