Fig. 5.

Wild-type M2r macrophage protect Was^−/−Rag^−/− mice from T-cell-transfer-induced colitis. Was^−/−Rag1^−/− mice were transfer with PBS (n = 9), WT M2r (2 × 10⁶) (n = 9) or Was^−/− M2r (2 × 10⁶) (n = 7) macrophages one-day before WT CD4⁺ T-cell transfer. As a control Rag1^−/− (n = 7) were treated with PBS and transferred with WT CD4⁺ T cells. a Mean ± SEM % initial body weight after T-cell transfer. Data are cumulative of two independent experiments. **p < 0.01, ***p < 0.001 (two-way ANOVA) (Was^−/−Rag1^−/− tx PBS Vs Was^−/−Rag1^−/− tx WT M2r). b Representative photomicrographs of H&E stained colonic section and histological score. Scale bars: 200 μm. c Absolute number and frequency of cytokine producing helper T cells in the LP was determined by flow cytometry. (Rag1^−/− tx PBS n = 5; Was^−/−Rag1^−/− tx PBS n = 5; Was^−/−Rag1^−/− tx WT M2r n = 5; Was^−/−Rag1^−/− tx Was^−/− M2r n = 5). Data are cumulative of two independent experiments. Data shown in b,c are mean ± SEM and P-value was obtained by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; NS, not significant; tx: treated