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. 2018 May 3;8:6958. doi: 10.1038/s41598-018-25198-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Representative color plot showing changes in current following 10 nM dopamine injection in PBS at t = 15 secs and 20 nM injection at t = 25 secs (in-vitro), (b) corresponding background subtracted CV showing reduction (−0.18 V) and oxidation (0.62 V) peaks of DA. Dopamine signals were measured using a standard pyramidal FSCV waveform in which the applied voltage was ramped from the holding potential of −0.5 V to the switching potential of +1.3 V and then back to −0.5 V at 400 V/s. (c) Background subtracted CVs for 10,20,100,200,500,700 nM and 1 μM DA concentration in PBS, showing reduction (−0.18 V) and oxidation (0.62 V) peaks, (d) Calibration curve corresponding to conditions in Fig. 9c. (e) Background subtracted CVs showing reduction (−0.18 V) and oxidation (0.62 V) peaks of 50 and 500 nM of DA in presence of 1 mM AA in PBS; (f) Calibration curve of the oxidation (0.6 V) peak of 50, 100, 200, 500, 700 nM and 1 μM DA concentration in 1 mM AA in PBS.