Figure 1.

HPAE-HPLC separation of labeled cellodextrin-(1→3)-d-Glc oligosaccharides from β-glucan synthesized in vitro. A, Separation of the β-glucan cellodextrin products synthesized at low substrate concentrations. Radioactivity from 0.5-mL fractions was determined by liquid scintillation spectroscopy after acidification of the NaOH with acetic acid. Upper trace, Pulsed amperometric detection of cellodextrin-(1→3)-d-Glc oligosaccharides from β-glucan. B, Separation of the β-glucan cellodextrin products synthesized at high substrate concentrations. The cellodextrin-(1→3)-d-Glc oligosaccharides from β-glucan made in vitro were quantified by pulsed amperometric detection (PAD).