Figure 6.

Immunodetection of SuSy in soluble (Sol), and plasma membrane (PM)- and Golgi membrane-enriched fractions of elongating maize coleoptiles and soybean hypocotyls. The Golgi membranes and soluble fraction were obtained after flotation centrifugation as described by Gibeaut and Carpita (1994), whereas the plasma membrane was obtained by two-phase aqueous partitioning. Ten micrograms of protein per lane was separated by SDS-PAGE in 10% (w/v) gels, and electroblotted onto cellulose nitrate by semidry blotting. SuSy was detected with polyclonal antisera at a 1:500 dilution for maize proteins and a 1:200 dilution for soybean proteins. Values are typical of four determinations.