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. 2018 May 3;8:6976. doi: 10.1038/s41598-018-25404-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of HSF1 truncation and internal deletion mutants on hsp70 promoter-reporter activity and HSF1 dimerization. (A) Expression of TAD-deleted HSF1 constructs inhibits endogenous HSF1 driven hsp70 promoter-reporter activity, while removal of the HR-A/B domain abrogates dominant negative activity. HEK293 cells were transfected with hsp70 promoter-reporter construct and HSF1 C-terminal truncation constructs, treated with DMSO or 17AAG for 1 hour, heat shocked at 42 °C for 30 minutes and allowed to recover for 4 hours before harvesting. (B) Disruption of HA-tagged HSP90α E47A interaction with HSF1 C-terminal RD truncations by 17AAG. HEK293 cells were transfected with HA-HSP90α E47A and FLAG-HSF1 RD truncation mutants, then analyzed by anti-FLAG (HSF1) IP followed by HA (HSP90α E47A) WB. Pull-down experiments were repeated at least twice. (C) Deletion of the DBD and retention of the HR-A/B domain inhibits hsp70-reporter activity driven by endogenous HSF1. HEK293 cells were transfected, treated and processed as described in panel A. (D) Deletion of the HR-A/B domain disrupts HSF1 dimerization. HEK293 cells were transfected with WT HA-HSF1 and FLAG-HSF1 deletion constructs, then analyzed by anti-FLAG IP and HA WB. Pull-down experiments were repeated at least twice. (E) Summary of the effects of HSF1 domain deletions on hsp70-reporter activity. Full blots used to create cropped figure panels are shown in Supplementary Figure 6.