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. 2018 May 3;8:6954. doi: 10.1038/s41598-018-25295-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Autophagosome formation is inhibited to a greater extent in WT-MMCs compared to miR-192-KO MMCs following TGF-β treatment. (A) Representative confocal microscopy images showing RFP-LC3 puncta in WT or miR-192-KO MMCs transfected with GFP-RFP-LC3 plasmid (1 µg) followed by TGF-β treatment of these cells for 24 hrs. x1000 (10 × 100 oil) magnification. (B) Bar graph showing quantification of RFP-LC3 puncta in WT and miR-192-KO MMCs. *P < 0.05; ***P < 0.001.