Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 27;9:775. doi: 10.3389/fimmu.2018.00775

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Meier-Soelch, Jurida, Weber, Newel, Kim, Braun, Schmitz and Kracht.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Differential effects of Sin3a knockdown on the expression of seven chemokine genes. Mef cells were transfected with pooled shRNAs directed against SIN3A (shSin3a) or with empty pLKO.1 (vector). After 48 h of selection in puromycin, cells were treated with IL-1 for the indicated times or were left untreated. (A) Expression of SIN3A was analyzed in total cell extracts by immunoblotting. Anti tubulin antibodies were used to confirm equal loading. (B) Total RNA was isolated and mRNA expression of the indicated chemokine genes and of Ube2l3 was determined by conventional RT-qPCR. Expression values were normalized for expression of Ube2l3. The graph show relative expression values ± SEM. from at least two independent experiments.