Table 1.
Summary of the features of HCV glycosylation mutants [adapted from Helle et al. (92)].
| Virus | HCVcc Infectivitya | HCVpp infectivitya,b | Core releasec | Sensitivity to neutralizationd |
|---|---|---|---|---|
| wt | +++ | +++ | ++ | + |
| Mutant | ||||
| E1N1 | +/− | ++ | − | ND (+) |
| E1N2 | ++ | + | + | ND (+) |
| E1N3 | +++ | ++ | ++ | ND (+) |
| E1N4 | ++ | + | +/− | ND (+) |
| E2N1 | +++ | ++ | ++ | ++ |
| E2N2 | ++ | − (−) | ++ | ++e |
| E2N3 | + | +++ | + | ND (+) |
| E2N4 | ++ | − (−) | + | ++e |
| E2N5 | ++ | ++ | ++ | + |
| E2N6 | +++ | ++ | ++ | ++ |
| E2N7 | +/− | +++ (+) | + | ND (−) |
| E2N8 | − | − | +/− | ND |
| E2N9 | +++ | +++ | ++ | + |
| E2N10 | − | − | − | ND |
| E2N11 | + | + | +/− | ++ |
| HVR495 | +++ | + | ND | ++ |
aPercentage of infectivity relative to the wild type (wt): +++, >90%; ++, between 30 and 90%; +, between 10 and 30%; +/−, between 2 and 10%; −, <2%.
bInfectivity of HCVpp of genotype 1a and 3a for the HVR495 glycan (9). The results in brackets are obtained for genotype 2a HCVpp.
cPercentage of core release relative to the wt: ++, >75%; +, between 30 and 75%; +/−, between 12 and 30%; −, <12%.
dSensitivity to antibody neutralization: +, similar to the wt; ++, more than fourfold increase in sensitivity to neutralization with most antibodies tested; −, decrease in sensitivity to neutralization. The values in brackets were obtained for genotype 1a HCVpp only.
eResults obtained with the HCVcc system only.
ND, not determined; HCV, hepatitis C virus; HCVpp, HCV pseudoparticles; HCVcc, cell culture-derived HCV.