Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 27;9:920. doi: 10.3389/fimmu.2018.00920

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Lee, Ko, Song and Jung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Lysophosphatidylcholine (LPC) enhances activation of phosphatidylinositol 3 kinase (PI3K)–p38 mitogen-activated protein kinase (MAPK) signaling and production of reactive oxygen species (ROS) and nitric oxide (NO) during Mycobacterium tuberculosis infection. (A) Raw264.7 cells were infected with H37Ra and treated with LPC for the indicated times. Phosphorylated and total protein levels for various MAPK signaling components were examined by Western blot analysis. (B) NO production was detected in cell culture supernatants at 24 h. (C) Intracellular ROS levels were measured based on dihydrodichlorofluorescein (DCF) fluorescence in LPC-treated wild-type (WT) and G2A knockout (KO) bone marrow-derived macrophages during H37Ra infection (multiplicity of infection of 5). (D) The bar graph represents the ratio of DCF fluorescence. (E) NO production was detected in cell culture supernatants at 24 h. The experiments were performed in triplicate (***p < 0.001).