Figure 8.

Lysophosphatidylcholine (LPC)-induced intracellular Ca²⁺ release and reactive oxygen species production regulate activation of NF-κB signaling and production of inflammatory cytokines by modulating phosphorylation of glycogen synthase kinase 3 beta (GSK3β) (Ser 9) in H37Ra-infected macrophages. (A) Raw264.7 cells were pretreated with BATPA/AM (30 µM) and stimulated with LPC during H37Ra infection (multiplicity of infection of 5). Whole-cell lysates were analyzed by Western blot analysis of the indicated proteins. (B) The production of TNF-α and IL-10 was measured 24 h post-infection in the presence or absence of BAPTA/AM (BAPTA) or apocynin (Apo) (*p < 0.05 and ***p < 0.001).