Figure 9.

Protein kinase-mediated signaling is involved in production of inflammatory cytokines by modulating phosphorylation of glycogen synthase kinase 3 beta (GSK3β) (Ser 9) in H37Ra-infected macrophages. (A) Raw264.7 cells were pretreated with H-89 for 1 h and then stimulated with lysophosphatidylcholine (LPC) during H37Ra infection [multiplicity of infection (MOI) of 5]. Production of TNF-α and IL-10 was measured in the cell culture medium at 24 h. (B) Whole-cell lysates were analyzed by Western blot analysis of the indicated proteins. (C) Raw264.7 cells were pretreated with SB216763 (10 µM) for 1 h and then stimulated with LPC during H37Ra infection (MOI of 5). Production of TNF-α and IL-10 was measured by enzyme-linked immunosorbent assay at 24 h. (D) Whole-cell lysates were analyzed by Western blot analysis of the indicated proteins; *p < 0.05; **p < 0.01; ***p < 0.001, and not significant (ns) (p > 0.05).