Changes in net O2 (A), 16O2 (B), and 18O2 concentration (C) in a closed system (approximately 150 mL) containing expanding (30- to 40-d-old) soybean seeds (dashed line, 0.976 g DW, n = 18; solid line, 1.219 g DW, n = 18; dotted line, 0.680 g DW, n = 15) exposed to either dark or light (1,000 μmol m−2 s−2) conditions. An approximate scale (μmol chamber−1) for all panels is provided in A. The initial O2 concentration was approximately 22% 18O2. Shaded areas indicate dark conditions.