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. 2018 Apr 27;9:841. doi: 10.3389/fimmu.2018.00841

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Copyright © 2018 Lee, Shin, Kim, Kim, Lee, Yoon, Uong, Yu, Jung, Cho, Jung, Kim and Suh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of the proliferative capacity in CD3⁻CD5⁻CD21⁻ non-B, non-T (CD5⁻), CD3⁺CD5^dimCD21⁻ (CD5^dim), and CD3⁺CD5^brightCD21⁻ (CD5^bright) lymphocytes. PBMCs were stained with Violet Cell Trace Dye before culture. To evaluate the proliferation of the different lymphocyte subsets, cells were stimulated with IL-15; IL-2; and IL-15; canine NK cell-sensitive canine thyroid adenocarcinoma (CTAC) cells; CTAC cells and IL-15; CTAC cells, IL-2, and IL-15; or concanavalin A for 7 days. Cells cultured in medium alone served as a negative control. Results are representative of data from five different donors. The percentage of proliferating cells within the respective subsets is indicated.