Table 1.
Antibodies used for flow cytometry in this study.
| Antibody | Clone | Conjugates | Supplier |
|---|---|---|---|
| Primary antibody (Isotype) | |||
| Mouse anti Human CD14 (IgG2a, κ)* | M5E2 | PE-Cy7 | BD Pharmingen |
| Mouse anti-Canine CD21 (IgG1) | CA2.1D6 | RPE | Bio-Rad |
| Mouse anti-Dog CD3 (IgG1) | CA17.2A12 | FITC | Bio-Rad |
| Rat anti-Dog CD5 (IgG2a) | YKIX322.3 | APC | Bio-Rad |
| Rat anti-Dog CD4 (IgG2a) | YKIX302.9 | RPE | Bio-Rad |
| Rat anti-Dog CD8 (IgG1) | YCATE55.9 | RPE | Bio-Rad |
| Mouse anti-Canine CD21 (IgG1) | CA2.1D6 | – | Bio-Rad |
| Mouse anti-Dog CD3 (IgG1) | CA17.2A12 | – | Bio-Rad |
| Mouse anti-Dog CD11c (IgG1) | CA11.6A1 | – | Bio-Rad |
| Mouse anti-Dog CD11d (IgG1) | CA11.8H2 | – | Bio-Rad |
| Canine TCRαβ (IgG1) | CA15.8G7 | – | Perter Moorea |
| Canine TCRγδ (IgG2a) | CA20.8H1 | – | Perter Moorea |
| Intracellular staining | |||
| Mouse anti Human Ki-67 (IgG1, kappa)* | 20Raj1 | PE-Cyanine7 | Invitrogen |
| Mouse anti Human Granzyme B (IgG1, κ)** | GB11 | PE | BD Pharmingen |
| Mouse anti Human EOMES (IgG1, kappa)b | WD1928 | PE-Cyanine7 | Invitrogen |
| Mouse anti Human/Mouse T-bet (IgG1, kappa)*** | eBio4B10 (4B10) | PE | Invitrogen |
| Isotype control | |||
| Mouse IgG1 Negative Control | – | FITC | Bio-Rad |
| Mouse IgG1 kappa Isotype Control | P3.6.2.8.1 | PE-Cyanine7 | Invitrogen |
| Mouse IgG1 kappa Isotype Control | P3.6.2.8.1 | PE | Invitrogen |
| Mouse IgG1, κ Isotype Control | MOPC-21 | PE | BD Pharmingen |
| Secondary antibody | |||
| Goat anti-Mouse IgG (H + L) Secondary Antibody | – | Pacific Blue | Invitrogen |