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. 2018 Apr 27;9:369. doi: 10.3389/fphar.2018.00369

FIGURE 2.

FIGURE 2

Evaluation of acute kidney injury (AKI). Tissue gene expression of the AKI markers Lcn-2, KIM-1, and serum creatinine (A). Values are expressed in relation to the housekeeping gene 18S for mRNA analyses and in mg/dl for creatinine measurements. All experiments were performed in duplicate. Representative micrographs of Lcn-2 stained kidney sections of untreated control, saline and Bβ15-42 treated mice at 24 and 48 h IR (B). Ten fields within the cortex and outer medulla in each group (n = 8) have been evaluated at 400-fold magnification. Quantification was performed using a script programmed in Matlab measuring proportional colored pixels in a defined relative area. Quantification was done by setting brown pixels into relationship to blue colored pixels. x significant difference to CTRL; f significant difference in the time course within Bβ15-42 treated or saline treated mice. x/f p < 0.05; xxp < 0.01; xxxp < 0.001.