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SPOCD1 promotes cell proliferation and metastasis in glioma by regulating PTX3. (A) PTX3 expression plasmid was utilized to re-express PTX3 in SPOCD1-depleted U87 cells. Western blot analysis was performed to confirm transfection efficiency (A). U87 cells transfected with a negative control shRNA (NC), shSPOCD1 and/or PTX3 expression plasmid were then subject to MTT assay (B), colony formation assay (C, D) and transwell migration assay (E, F).
