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Inhibition of autophagy attenuates upregulation of CDKI and cell cycle arrest caused by GGCT knockdown. (A) Western blot analysis of p21, p16, GGCT, Atg5, and GAPDH expression in MCF7 and PC3 cells at 4 days post-transfection with siRNA targeting GGCT or with a non-target control siRNA. (B and C) Cell cycle phases in MCF7 cells were analyzed by flow cytometry at 4 days post-transfection with the indicated siRNAs. Representative histograms (B) and quantified distribution are shown (C).
