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. 2018 Apr 1;8(4):650–661.

Figure 6.

Figure 6

Depletion of GGCT activates AMPK-ULK1 pathway and/or leads to inactivation of the mTORC2-Akt in cancer cells. A: Western blot analyses of mediators of the AMPK-ULK1 signaling pathway (p-AMPKα Thr172, AMPKα, p-ULK1 Ser555, and ULK1) and downstream components of the mTORC1 pathway (p-ULK1 Ser757 and p-p70S6K Thr389) in MCF7 and PC3 cells 4 days post-transfection with siRNA targeting GGCT or with a non-target control siRNA. GAPDH and β-tubulin are shown as loading controls. B: Western blot analyses of p-Rictor Thr1135, Rictor, p-Akt Ser473, Akt, GAPDH and β-tubulin in MCF7 and PC3 cells 4 days post-transfection with the indicated siRNAs are shown.