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. 2018 Apr 1;8(4):662–674.

Figure 4.

Figure 4

JAK2 was a direct target gene of miR-337-3p. A. TargetScan, miRDB, and PicTar databases were used to predict the target genes of miR-337-3p, and JAK2 was predicted by all three databases. B. The putative binding sequence of miR-337-3p in the 3’-UTR of JAK2. C. The dual luciferase reporter assay showed that miR-337-3p bound to the 3’-UTR of JAK2 (P < 0.01). D. qRT-PCR analysis of JAK2 mRNA expression in 50 pairs of HCC and peritumor tissues. The expression of JAK2 was obviously increased in HCC tissues compared with the peritumor tissues (P < 0.001). E. A significant inverse correlation between miR-337-3p and JAK2 mRNA was observed in HCC tissues (P = 0.002). F. Overexpression of miR-337-3p reduced the expression of JAK2 and p-STAT3 in MHCC-LM3 and SMMC7721 cells. Data are shown as mean ± SD. **P < 0.01, ***P < 0.001.