Figure 5.

Influence of bicalutamide or hormone-depleted media on T-type Ca²⁺ channels in regulating the neurite-like morphology of LNCaP cells. (A, B) Effect of Ni²⁺ ions (NiCl₂, 100 µM) or NNC 55-0396 (5 μM) on total neurite-like length (A) and average number of primary processes per cell (B) in LNCaP cells stimulated with bicalutamide or hormone-depleted media for 10 d. Stimulation of LNCaP cells with bicalutamide or hormone-depleted media for 10 d evoked a significant increase in total neurite-like length (A) and average number of primary processes per cell (B) compared to non-stimulated cells. Inhibition of T-type Ca²⁺ channels with Ni²⁺ ions or NNC 55-0396 evokes a significant decrease in the total neurite-like length and average number of primary processes per cell. The number of cells analyzed under each condition is represented above each bar (*P<0.05 vs. non-stimulated control cells; **P<0.05 vs. bicalutamide-stimulated cells; ***P<0.05 vs. treatment with hormone-depleted media; ns, P>0.05 vs. bicalutamide-stimulated cells).