Table 1.
Sequences of the primers and fluorescent probes
| Sequence | ||
|---|---|---|
| Type II Collagen | Sense | GAGGTGGATGCCACACTCAAG |
| Antisense | CCACTCAGGATGGCAGAGTTTC | |
| Taqman probe | AACCAGATTGAGAGCATCCGCAGCC | |
| CTGF | Sense | GGCAAAAAGTGCATCCGTACTC |
| Antisense | ATCGGCCGTCGGTACATACTC | |
| Taqman probe | TTTGAGCTTTCTGGCTGCACCAGCA | |
| TIMP-1 | Sense | GCTTCTGGCATCCTGTTGTTG |
| Antisense | CTTCTGGTGTCCCCACGAACT | |
| Taqman probe | AGACGGCCTTCTGCAATTCCGACCT | |
| Aggrecan | Sense | GGACTTCCGCTGGTCAGATG |
| Antisense | GGCGGCAAAGAAGTTGTCA | |
| Taqman probe | ATTTGAGAACTGGCGCCCCAACCA | |
Upstream and downstream primers were synthesized by the Shanghai Bioengineering Technology Service Co., Ltd. (Shanghai, China) Fluorescent probes were synthesized by the Dalianbao Biological Company (Dalian, China).