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Up-regulated expression of miR-520d-3p promotes cells apoptosis. A. Flow cytometry analysis of apoptosis. After 24 h treatment, transfection of miR-520d-3p mimics into MCF-7 and T47D cell lines resulted in a significant increase percentage of apoptotic cells compared with the control. B. Expression level of Bax and Bcl-2 was determined by western blotting, using GAPDH as a control. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
