Table 1.
Estimated photon flux by microscope
| Microscope | Light intensity (nW/µm2) | Photon flux (photons/µm2 per 3D image) |
|---|---|---|
| Widefield | GFP: 0.15–0.4 | 2.7×109–1.1×1010 |
| RFP: 0.27–0.75 | ||
|
| ||
| Spinning disk | 488: 6.7–17.0 | 8.0×1010–2.5×1011 |
| 561: 4.2–10.0 | ||
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