Figure 7.

Ectopic expression of eIF4A in viral factories reduced SV mRNA translation. (A) Exclusion of eIF4A from SFV viral factories. IF of eIF4A in mock and SFV-infected cells, with SFV C protein stained in red and eIF4A in green. Note the exclusion of eIF4A from viral factories as revealed by C protein staining (arrowheads). About 75% of analyzed cells showed this pattern, and a representative field is shown. (B) Schematic representation of recombinant SV expressing human eIF4A-myc. Cells were infected with two independent SV-eIF4A clones and analyzed by western blot against myc epitope (right upper panel), by IF against SV C and myc epitopes (left lower), and by metabolic labeling with [³⁵S]-Met. The position of viral protein bands is indicated; in all cases, samples were analyzed at 6 hpi, with two SV clones expressing EGFP used as controls.