Table 1. Synthetic oligonucleotides (in complementary pairs) used for crystallography and UNG activity assays.

5′FAM-idu_2 (crystallography)	FAM-5′-AAAGATAACATT-3′
	3′- TTCTAUTGTAA-5′
5′-FAM-U	FAM-5′- ATATATAUATATATAACGCAGACACGTAGCAC-3′
5′-G-BHQ1 (U:G duplex for ung assay)	BHQ1–3′-TATATATATATGTATATATTGCGTCTGTGCATCGTG-5′
5′-FAM-U	FAM-5′- ATATATAUATATATAACGCAGACACGTAGCAC-3′
5′-A-BHQ1 (U:A duplex for ung assay)	BHQ1–3′-TATATATATATATATATATTGCGTCTGTGCATCGTG-5′

FAM-5′ refers to the presence of a covalently linked 6-FAM label at the 5′ end of the oligonucleotide. BHQ1 refers to a covalently linked Black Hole Quencher 1 label at the 3′ end of the oligonucleotide. Uracil is shown in bold and underlined.