. 2018 Mar 27;46(8):4286–4300. doi: 10.1093/nar/gky217

Table 3. Enzymatic parameters of wild-type and mutant kUNG using U:G and U:A duplexes.

Enzymatic Parameters^a	WT	Δ insYRG	Q212E	R229A	R223Q	R223S
U:G duplex
V _max (RFU s⁻¹)	0.857 ± 0.021	0.580 ± 0.017	0.718 ± 0.024	0.820 ± 0.017	0.857 ± 0.018	0.866 ± 0.017
K _m (nM)	48.870 ± 4.056	101.100 ± 7.463	63.007 ± 6.463	45.240 ± 3.323	47.450 ± 3.486	47.510 ± 3.148
U:A duplex
V _max (RFU s⁻¹)	0.872 ± 0.020	0.396 ± 0.018	0.153 ± 0.005	0.954 ± 0.022	0.899 ± 0.029	0.851 ± 0.025
K _m (nM)	44.99 ± 3.609	270.6 ± 22.42	145.4 ± 10.64	86.24 ± 5.464	162.6 ± 11.25	140.9 ± 9.494

^a V _max and K_m was determined by FRET-based assay. Values (mean ± SE) are the result of between 4 and 12 measurements. Data was analysed using a non-linear regression fit and the Michaelis–Menten equation (least squares fit).