Table 2. NMR restraints and structural statistics for the M2 G-quadruplex in the absence of cosolutes.
| NOE-derived distance restraints | Non-exchangeable | Exchangeable |
|---|---|---|
| Intranucleotide NOEs | 323 | 0 |
| Sequential (i, i+1) | 64 | 8 |
| Long-range (i, > i+1) | 10 | 22 |
| Torsion angle restraints | 97 | |
| Hydrogen-bond restraints | 24 | |
| Structural statistics | ||
| NOE violations >0.2Å | 0 | |
| Deviations from idealized covalent geometry | ||
| Bonds (Å) | 0.011 ± 0.000 | |
| Angles (°) | 2.246 ± 0.064 | |
| Pairwise heavy atom RMSD (Å) | ||
| Overall | 0.890 | |
| Without 5′ TA overhang | 0.894 | |
| Without 3′ T overhang | 0.902 | |
| Without 5′ TA and 3′ T overhangs | 0.907 | |
| Without A6, C11 and C15, A16 loop residues | 0.929 | |
| Only core of the structure (only guanine residues) | 0.510 | |