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Genomic SELEX patterns of group-S2 single-target TFs. In the absence of effectors, BetI and NanR bound to a number of non-specific sites (A1 and B1). In the presence of specific effectors (choline for BetI and NANA for NanR), both BetI and NanR bound only to the respective specific target site, but all the non-specific binding disappeared (A2 and B2). TFs showing this type of binding specificity are classified into group-S3 single-target TFs.
