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. 2018 Feb 26;46(8):3921–3936. doi: 10.1093/nar/gky138

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Control of the activity of BetI by two effectors. Activity control of BetI by KCl and choline. Gel shift assay was performed to observe the influence of KCl and choline, the predicted effectors of BetI, on the BetI activity. In the presence of 0.5 M KCl, the binding of Bet was observed only at the spacer between betI and betT (A and D), the specific single-target of BetI, but non-specific binding inside ORFs of ybjE (B and E) and srmB (C and F) genes disappeared. Details of the gel shift assay are described in the ‘Materials and Methods’ section. The concentration of choline added was: 0 (lane 2), 10 nM (lane 3), 100 nM (lane 4); 1 μM (lane 5); 10 μM (lane 6); 100 μM (lane 7); 1 mM (lane 8); 10 mM (lane 9); 100 mM (lane 10).